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Skin PAMPA Permeability System

Uploader: Gardakinos 4 years ago  
3788 views
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The validation of the method has been put on hold, as the method will be considered to be followed up in the wider context of ADME methods. Therefore, passive diffusion can be determined at the pH values observed in the gastrointestinal tract. Rodent in vivo and in situ studies of the kinetics of drug uptake across the BBB are valuable tools for assessing factors important to steady-state brain penetration. These are relatively expensive and time consuming assays to do, and are done only sparingly. Method Description The Skin PAMPA is a method that measures the passive permeation of a substance, applied in solution or as a formulation, through an artificial membrane mimicking the human skin. Lucifer yellow is used to assess membrane integrity.

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